Journal: Scientific Reports

Article Title: DF2726A, a new IL-8 signalling inhibitor, is able to counteract chemotherapy-induced neuropathic pain

doi: 10.1038/s41598-019-48231-z

Figure Lengend Snippet: Western blotting analyisis for microglia marker Iba-1 and for astrocyte GFAP in control, OXA and (Oxa) + DF2726A rats. In the bottom, TRPA1 and TRPM8 receptors in in control, OXA and (Oxa) + DF2726A rats, evalutated by western blotting. Administration of DF2726A (Oxa + DF, light grey triangles) was able to significantly reduce reactive microglia and astrocytes as well as to reduce pain receptors. ***P < 0.001, **P < 0.01 and *P < 0.05 vs respective CTR group; +++ P < 0.001; ++ P < 0.01 and + P < 0.05 vs the respective Oxa + Saline group. 2-way repeated-measures ANOVA and Bonferroni test. Data are expressed as mean ± SEM; n 10 per group. ANOVA, analysis of variance.

Article Snippet: The filter was then blocked with 1 × PBS and 3% non-fat dried milk for 40 min at room temperature and probed with anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluition 1.1000; cat.no.Z0334, Dako), anti-ionized calcium-binding adapter molecule 1 (Iba-1) antibody (dilution 1:1000; cat. no.019-19741, Wako), anti-transient receptor potential ankyrin 1 (TRPA1) antibody (diluition 1:1000; cat. no. NB110-40763, Novus Biologicals) and anti- Transient receptor potential M8 (TRPM8) antibody (diluition 1:10100; cat. no. NBP1-97311, Novus Biologicals) in 1 × PBS, 3% non-fat dried milk, and 0.1% Tween 20 at 4 °C overnight.

Techniques: Western Blot, Marker, Control, Saline

Journal: Scientific Reports

Article Title: Characterization of human iPSC-derived sensory neurons and their functional assessment using multi electrode array

doi: 10.1038/s41598-024-55602-8

Figure Lengend Snippet: Expression of sensory neuron related genes in hiPSC, hiPSC-derived sensory neurons and human DRG. Real-time PCR showed expression of ( a ) Peripherin, ( b ) Brn3a, ( c ) TRPV1, ( d ) TRPA1, ( e ) TRPM8, ( f ) Nav1.7, ( g ) Nav1.8, ( h ) Piezo2, ( i ) TRKA, ( j ) TRKB, ( k ) TRKC, ( l ) P2X3, ( m ) H1R, ( n ) MrgprX1, ( o ) CGRP, ( p )TAC1. The square marker, the circle marker and triangle marker indicate expression of genes in hiPSC, hiPSC-derived sensory neurons and human DRG respectively. Three different lot of hiPSC-derived sensory neurons were examined. The line marker represents the mean expression of genes in hiPSC-derived sensory neurons.

Article Snippet: Fixed samples were incubated with primary antibodies against TUBB3 (1:1000, Covance, PRB-435P), Brn3a (1:25, Millipore, MAB1585), Peripherin (1:200, Millipore, AB1530), TRPV1 (1:100, Invitrogen, PA1-748), TRPM8 (1:100, NOVUS, NBP1-97311), Nav1.7 (1:100, NOVUS, NBP2-12904), TRKA (1:80, R&D SYSTEMS, MAB1751R), TRKB (1:80, R&D SYSTEMS, MAB3971), TRKC (1:100, R&D SYSTEMS, AF373), Neurofilament 200 (1:1000, Sigma, N4142), Isolectin B4 (1:1000, Thermo Fisher Scientific, I21411) over night at 4 °C.

Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Marker

Journal: Scientific Reports

Article Title: Characterization of human iPSC-derived sensory neurons and their functional assessment using multi electrode array

doi: 10.1038/s41598-024-55602-8

Figure Lengend Snippet: Expression of sensory neuron related proteins in hiPSC-derived sensory neurons and their morphology. The cells are stained for ( a ) TUBB3, ( b ) Peripherin, ( c ) Brn3a, ( d ) TRPV1, ( e ) TRPM8, ( f ) Nav1.7, ( g ) TRKA, ( h ) TRKB, ( i ) TRKC, ( j ) NF200, ( k ) IB4. DAPI stain of nuclei is shown in blue. ( l ) Image of iPSC-derived sensory neurons which exhibit a bipolar (red arrowhead), pseudounipolar (yellow arrowhead), or multipolar morphology (green arrowhead). Scale bar represents 50 µm.

Article Snippet: Fixed samples were incubated with primary antibodies against TUBB3 (1:1000, Covance, PRB-435P), Brn3a (1:25, Millipore, MAB1585), Peripherin (1:200, Millipore, AB1530), TRPV1 (1:100, Invitrogen, PA1-748), TRPM8 (1:100, NOVUS, NBP1-97311), Nav1.7 (1:100, NOVUS, NBP2-12904), TRKA (1:80, R&D SYSTEMS, MAB1751R), TRKB (1:80, R&D SYSTEMS, MAB3971), TRKC (1:100, R&D SYSTEMS, AF373), Neurofilament 200 (1:1000, Sigma, N4142), Isolectin B4 (1:1000, Thermo Fisher Scientific, I21411) over night at 4 °C.

Techniques: Expressing, Derivative Assay, Staining